The Group B encounter was played at the Stade du 4 Août in Ouagadougou.
Oluwakemi Adegbuyi opened the scoring in the 23rd minute with a powerful header. Tarnum Dooshima doubled the lead just before half-time in the 41st minute, before Oluebube Umejiaku added a third goal in the 89th minute to seal the win.
ASEC Mimosas scored a late consolation goal but were unable to mount a serious recovery.
The result gives Edo Queens a second consecutive victory in the tournament after their earlier 7-0 thrashing of AS GNN. The Nigerian side remain top of Group B and are well placed to progress further in the qualifiers for the CAF Women’s Champions League.