The Group A4 fixture kicks off at 19:45 local time (18:45 GMT / 19:45 WAT) at the Estádio José Alvalade in Lisbon.
Cristiano Ronaldo is expected to start, with Jesus confirming the 41-year-old will lead the line. Ronaldo, who has scored 146 international goals, remains a central figure in the squad despite speculation about his future after Portugal’s World Cup exit.
Predicted Portugal Line-up
Diogo Costa; João Cancelo, Rúben Dias, Renato Veiga, Nuno Mendes; Vitinha, João Neves; Pedro Neto, Bruno Fernandes, João Félix; Cristiano Ronaldo
Jesus has a largely full-strength squad available for his debut. Key players such as Vitinha, Bruno Fernandes and Rúben Dias are all expected to feature from the start.
Wales Team News
Wales head coach Craig Bellamy is without several players through injury. Harry Wilson, Nathan Broadhead and Karl Darlow have withdrawn from the squad, while Joe Rodon, Dylan Lawlor, Rubin Colwill and Isaak Davies were already absent.
Predicted Wales Line-up
Danny Ward; Neco Williams, Ben Cabango, Ben Davies / Chris Mepham, Jay DaSilva; Ethan Ampadu, Jordan James; David Brooks, Sorba Thomas, Brennan Johnson; Daniel James
Wales return to League A after promotion and will look to frustrate the hosts on their first competitive outing since the World Cup play-offs.
Portugal, as defending Nations League champions, begin their title defence under a new coach following Roberto Martinez’s departure after the World Cup. The match marks the start of a new cycle for the Seleção with Jesus at the helm.